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KMID : 0370220110550060478
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Yakhak Hoeji
2011 Volume.55 No. 6 p.478 ~ p.484
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Inhibitory Effect of Triticum aestivum Ethanol Extract on Lipid Accumulation in 3T3-L1 Preadipocytes
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Lee Sun-Hee
Xin Mingjie
Luyen Bui Thi Thuy
Cha Ji-Yun
Im Ji-Young
Kwon Se-Uk
Lim Sung-Won
Kim Young-Ho
Kim Dae-Ki
Lee Young-Mi
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Abstract
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Non-alcoholic fatty liver disease is known to be frequently associated with obesity and type 2 diabetes. We examined the effects of EtOH extracts from Triticum aestivum on lipid accumulation during the differentiation of 3T3-L1 preadipocytes to screening the candidate materials in preventing non-alcoholic fatty liver disease. The lipid level in adipocytes was determined by Oil Red O staining. The treatment of 50% ethanol, but not water and 100% ethanol extracts, from Triticum aestivum at concentration of 0.5 inhibited lipid accumulation in 3T3-L1 cells, revealing no cell toxicity. Thus, the fractions of , EtOAc and BuOH were separated from 50% EtOH extract to characterize anti-adipogenic effect. The fraction at concentration of effectively inhibited the lipid accumulation in the adipocytes compared to those of EtOAc and BuOH at concentration of . The intracellular triglyceride accumulation also was significantly reduced by treatment of fraction in concentration-dependent manner. Western blot analysis showed that the fraction attenuated the intracelluar level of fatty acid synthase(FAS) accompanied by attenuated expression of Peroxidase proliferator-activated receptor () adipogenic transcription factor. These results suggest that fraction from 50% EtOH extract of Triticum aestivum may has the potent anti-adipogenic effects by inhibiting the transactivation of .
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KEYWORD
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Triticum aestivum, 3T3-L1 preadipocytes, lipid accumulation, fatty acid synthase, anti-adipogenesis
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